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iD (mA) Ohmic vDS > vGS VP Constant current vGS VP vGS = 3 V 20 2V 16 1V 12 8 0V 4 1 V 0 2 V 3 V 4 V 0 4 8 12 16 20 vDS (V)

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Ultraviolet (UV) light causes linkage, or dimerization, of adjacent pyrimidines in DNA ( g. 12.27). Although cytosine-cytosine and cytosine-thymine dimers are occasionally produced, the principal products of UV irradiation are thymine-thymine dimers. These can be repaired in several different ways. The simplest is to reverse the dimerization process and restore the original unlinked thymines. In E. coli, an enzyme called DNA photolyase, the product of the phr gene (for photoreactivation), binds to dimerized thymines. When light shines on the cell,

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as we increase the gate-source voltage, the transistor acts as a variable resistor, dependent on the gate-source voltage This corresponds to the ohmic region of operation of the depletion-mode MOSFET, depicted in Figure 937(c) As the drain-source voltage is increased for a xed gate-source voltage, the relative strength of the eld will decrease near the drain end of the device, until the channel is pinched off When the pinch-off condition is reached, the transistor acts as a constant-current source, and is in the saturation region This condition is depicted in Figure 937(d) In effect, a depletion-mode MOSFET acts very much like an enhancement mode device with a negative threshold voltage Unlike an enhancement MOSFET, however, the depletion MOSFET

the enzyme breaks the dimer bonds with light energy. The enzyme then falls free of the DNA.This enzyme thus reverses the UV-induced dimerization. Another example of an enzyme that performs direct DNA repair is O6mGua DNA methyltransferase, which removes the methyl groups from O6-methylguanine, the major product of DNA-methylating agents (fig. 12.28). Although other repair mechanisms seem to be present in all organisms, photoreactivation is not; it is apparently absent in human beings.

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Figure 938 depicts the characteristic curves of a depletion-mode MOSFET Once again, note the similarity to the enhancement-MOSFET curves: The primary difference results from the fact that, since a channel exists even for zero gate voltage, the device can operate for both positive and negative gate-source voltages Junction Field-Effect Transistors The last member of the FET family that will be discussed in this chapter is the junction eld-effect transistor (JFET) The construction of a JFET and its circuit

Fig 48: (a) Different sizes of Winter diamond-grinding pins that are normally used for internal grinding operations

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Excision repair refers to the general mechanism of DNA repair that works by removing the damaged portion of a DNA molecule. Various enzymes can sense damage or distortion in the DNA double helix. During excision repair, bases and nucleotides are removed from the damaged strand. The gap is then patched using complementarity with the remaining strand. We can broadly categorize these systems as base excision repair and nucleotide excision repair, which includes mismatch repair. We will discuss only the major repair pathways; others exist. Presumably, redundancy in repair has been selected for because of the critical need to keep DNA intact and relatively mutation free.

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